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Journal of Bacteriology, April 2009, p. 2392-2399, Vol. 191, No. 7
0021-9193/09/$08.00+0     doi:10.1128/JB.01397-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Creation of a Large Deletion Mutant of Campylobacter jejuni Reveals that the Lipooligosaccharide Gene Cluster Is Not Required for Viability{triangledown}

Gemma L. Marsden,1,{dagger} Jianjun Li,2 Paul H. Everest,3 Andrew J. Lawson,4 and Julian M. Ketley1*

Department of Genetics, University of Leicester, Leicester, United Kingdom,1 Institute of Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada,2 Institute of Comparative Medicine, Sir Henry Wellcome Building, University of Glasgow Faculty of Veterinary Medicine, Glasgow, United Kingdom,3 Laboratory of Enteric Pathogens, Health Protection Agency Centre for Infections, London, United Kingdom4

Received 6 October 2008/ Accepted 15 January 2009

Deletion of the lipooligosaccharide biosynthesis region (Cj1132c to Cj1152c) from the genome of Campylobacter jejuni NCTC11168 shows that the core is not required for viability. The mutant was attenuated for growth and has increased sensitivity to antibiotics and detergents. Natural transformation and invasion of cultured host cells was abolished.

* Corresponding author. Mailing address: Department of Genetics, University of Leicester, University Road, Leicester, United Kingdom. Phone: 44 (0)116 252 3434. Fax: 44 (0)116 252 3378. E-mail: ket{at}le.ac.uk

{triangledown} Published ahead of print on 30 January 2009.

{dagger} Present address: Clostridial Research Group, Institute of Infection, Immunity and Inflammation, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom.

Journal of Bacteriology, April 2009, p. 2392-2399, Vol. 191, No. 7
0021-9193/09/$08.00+0     doi:10.1128/JB.01397-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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