This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gibb, E. A.
Right arrow Articles by Edgell, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gibb, E. A.
Right arrow Articles by Edgell, D. R.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2009, p. 2409-2413, Vol. 191, No. 7
0021-9193/09/$08.00+0     doi:10.1128/JB.01751-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

An RNA Hairpin Sequesters the Ribosome Binding Site of the Homing Endonuclease mobE Gene{triangledown}

Ewan A. Gibb and David R. Edgell*

Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada N6A 5C1

Received 15 December 2008/ Accepted 21 January 2009

Previous transcript mapping of the bacteriophage Aeh1 nrd operon revealed a predicted RNA hairpin upstream of the homing endonuclease mobE gene. We enzymatically mapped the hairpin, showing that the mobE ribosome binding site is sequestered. Cloning of the hairpin upstream of lacZ resulted in reduced β-galactosidase activity, consistent with translational regulation.

* Corresponding author. Mailing address: The University of Western Ontario, Department of Biochemistry, London, Ontario, Canada N6G 1C7. Phone: 519-661-3133. Fax: 519-661-3175. E-mail: dedgell{at}uwo.ca

{triangledown} Published ahead of print on 30 January 2009.

Journal of Bacteriology, April 2009, p. 2409-2413, Vol. 191, No. 7
0021-9193/09/$08.00+0     doi:10.1128/JB.01751-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»