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Journal of Bacteriology, April 2009, p. 2541-2550, Vol. 191, No. 8
0021-9193/09/$08.00+0     doi:10.1128/JB.01695-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

BldG and SCO3548 Interact Antagonistically To Control Key Developmental Processes in Streptomyces coelicolor{triangledown}

Archana Parashar, Kimberley R. Colvin, Dawn R. D. Bignell,{dagger} and Brenda K. Leskiw*

Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada

Received 4 December 2008/ Accepted 31 January 2009

The similarity of BldG and the downstream coexpressed protein SCO3548 to anti-anti-sigma and anti-sigma factors, respectively, together with the phenotype of a bldG mutant, suggests that BldG and SCO3548 interact as part of a regulatory system to control both antibiotic production and morphological differentiation in Streptomyces coelicolor. A combination of bacterial two-hybrid, affinity purification, and far-Western analyses demonstrated that there was self-interaction of both BldG and SCO3548, as well as a direct interaction between the two proteins. Furthermore, a genetic complementation experiment demonstrated that SCO3548 antagonizes the function of BldG, similar to other anti-anti-sigma/anti-sigma factor pairs. It is therefore proposed that BldG and SCO3548 form a partner-switching pair that regulates the function of one or more sigma factors in S. coelicolor. The conservation of bldG and sco3548 in other streptomycetes demonstrates that this system is likely a key regulatory switch controlling developmental processes throughout the genus Streptomyces.

* Corresponding author. Mailing address: Department of Biological Sciences, CW405 Biological Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2E9. Phone: (780) 492-2076. Fax: (780) 492-7033. E-mail: brenda.leskiw{at}ualberta.ca

{triangledown} Published ahead of print on 6 February 2009.

{dagger} Present address: Department of Plant Pathology and Plant-Microbe Biology, 334 Plant Science Building, Cornell University, Ithaca, NY 14853.

Journal of Bacteriology, April 2009, p. 2541-2550, Vol. 191, No. 8
0021-9193/09/$08.00+0     doi:10.1128/JB.01695-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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