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Journal of Bacteriology, April 2009, p. 2675-2682, Vol. 191, No. 8
0021-9193/09/$08.00+0     doi:10.1128/JB.01814-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

A Molybdopterin Oxidoreductase Is Involved in H2 Oxidation in Desulfovibrio desulfuricans G20{triangledown} ,{dagger}

Xiangzhen Li,1 Qingwei Luo,1,{ddagger} Neil Q. Wofford,1 Kimberly L. Keller,2 Michael J. McInerney,1 Judy D. Wall,2 and Lee R. Krumholz1,3*

Department of Botany and Microbiology,1 Institute for Energy and the Environment, The University of Oklahoma, Norman, Oklahoma 73019,3 Department of Biochemistry, University of Missouri, Columbia, Missouri 652112

Received 23 December 2008/ Accepted 11 February 2009

Three mutants deficient in hydrogen/formate uptake were obtained through screening of a transposon mutant library containing 5,760 mutants of Desulfovibrio desulfuricans G20. Mutations were in the genes encoding the type I tetraheme cytochrome c3 (cycA), Fe hydrogenase (hydB), and molybdopterin oxidoreductase (mopB). Mutations did not decrease the ability of cells to produce H2 or formate during growth. Complementation of the cycA and mopB mutants with a plasmid carrying the intact cycA and/or mopB gene and the putative promoter from the parental strain allowed the recovery of H2 uptake ability, showing that these specific genes are involved in H2 oxidation. The mop operon encodes a periplasm-facing transmembrane protein complex which may shuttle electrons from periplasmic cytochrome c3 to the menaquinone pool. Electrons can then be used for sulfate reduction in the cytoplasm.

* Corresponding author. Mailing address: Department of Botany and Microbiology, The University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019. Phone: (405) 325-0437. Fax: (405) 325-7619. E-mail: krumholz{at}ou.edu

{triangledown} Published ahead of print on 20 February 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Department of Biology, Campus Box 1137, Washington University in St. Louis, One Brookings Dr., St. Louis, MO 63130.

Journal of Bacteriology, April 2009, p. 2675-2682, Vol. 191, No. 8
0021-9193/09/$08.00+0     doi:10.1128/JB.01814-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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