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J Bacteriol. 1962 January; 83(1): 175-181
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.

INFLUENCE OF GUINEA PIG PLASMA FACTORS ON PHAGOCYTOSIS OF PASTEURELLA PESTIS

I. Plasma From Normal Guinea Pigs1

W. G. Stanziale2 and J. D. White

a U. S. Army Chemical Corps Biological Laboratories, Fort Detrick, Frederick, Maryland

ABSTRACT

STANZIALE, W. G. (Fort Detrick, Frederick, Md.) AND J. D. WHITE. Influence of guinea pig plasma factors on phagocytosis of Pasteurella pestis. I. Plasma from normal guinea pigs. J. Bacteriol. 83:175–181. 1962.—The existence of an enhancing effect of normal guinea pig plasma on the phagocytosis of Pasteurella pestis has been reaffirmed, using a test system consisting of neutrophils from normal nonimmune guinea pigs as the phagocytes. This enhancing influence was found to be attributable to the interplay of a thermolabile and a thermostable plasma component.

The data suggest a consideration of complement as the thermolabile component, since the enhancing activity of plasma is destroyed by heating to 56 C and restored by addition of fresh plasma. The possibility that the thermolabile plasma component is opsonin was discounted. Surface alterations which did occur resulted in preserving the integrity of the envelope substance of the plague bacillus. Treatment of neutrophils with plasma prior to phagocytosis did not increase their phagocytic capacity.

The thermostable enhancing component was not demonstrated to be present in any of three purified serum protein fractions tested. In fact, all of these fractions inhibited phagocytosis of P. pestis. An enhancing effect of purified fibrinogen could not be demonstrated.

FOOTNOTES

2 Present address: Department of Biology, St. Joseph College, West Hartford, Conn.

1 From a dissertation submitted by the senior author to the Graduate School of Arts and Science, The Catholic University of America, Washington, D. C., in partial fulfillment of requirements for the degree of Doctor of Philosophy.

J Bacteriol. 1962 January; 83(1): 175-181
Copyright © 1962, The Williams & Wilkins Company. All Rights Reserved.





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