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J Bacteriol. 1966 November; 92(5): 1298-1303
Copyright © 1966 American Society for Microbiology. All Rights Reserved.

Antigenic Structure of Brucella suis Spheroplasts1

Robert E. Baughn and Bob A. Freeman

a Department of Microbiology, University of Tennessee Medical Units, Memphis, Tennessee

ABSTRACT

BAUGHN, ROBERT E. (University of Tennessee, Memphis), AND BOB A. FREEMAN. Antigenic structure of Brucella suis spheroplasts. J. Bacteriol. 92:1298–1303. 1966.—Immunoelectrophoresis was used to differentiate between the antigenic mosaics of normal cells of Brucella suis and of spheroplasts prepared by treatment with penicillin, glycine, and a combination of these agents. Smooth cells possessed at least 13 antigens, 10 of which were precipitated with homologous antiserum. Three additional antigens were visualized by reaction with spheroplast antisera. Spheroplasts induced with glycine were the least complex, with only six antigens. Penicillin-glycine spheroplasts were similar, but possessed one additional antigen. Penicillin spheroplasts were the most complex, with eight antigens. Although there appeared to be quantitative differences between the antigens of spheroplasts and normal cells, no completely new antigens were detected in spheroplasts. Serum absorption studies indicated that four antigens were associated with the surface of normal B. suis, none of which occurred in spheroplasts.

FOOTNOTES

1 The data in this paper were taken from a thesis submitted by R. E. Baughn in partial fulfillment of the requirements for the M.S. degree from the University of Tennessee, Medical Units.

J Bacteriol. 1966 November; 92(5): 1298-1303
Copyright © 1966 American Society for Microbiology. All Rights Reserved.





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