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J Bacteriol. 1967 February; 93(2): 703-710
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Occurrence of Isocitrate Lyase in a Thermophilic Bacillus Species

Harlow H. Daron

Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas

ABSTRACT

A thermophilic, sporeforming bacterium has been isolated from soil on a medium containing acetate as a carbon source. This organism is similar to Bacillus stearothermophilus in most respects but differs in its inability to hydrolyze starch. Isocitrate lyase is present in cell-free extracts of organisms grown in a medium with acetate as a carbon source. The specific activity was 400 times lower in extracts of organisms utilizing glucose as a carbon source. With crude extracts, enzyme activity was strongly stimulated by Mg++, but cysteine and ethylenediaminetetraacetate had little effect. It appeared to be more heat-stable than the pure isocitrate lyase from Pseudomonas indigofera.

J Bacteriol. 1967 February; 93(2): 703-710
Copyright © 1967 American Society for Microbiology. All Rights Reserved.





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