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J. Bacteriol. doi:10.1128/JB.00019-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The Escherichia coli regulator of sigma 70 protein, Rsd, can up-regulate some stress-dependent promoters by sequestering sigma 70

School of Biosciences, University of Birmingham, Birmingham B15 2TT, U.K.; Nara Institute of Science and Technology, Nara 630-0101, Japan; The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA; Institut Pasteur, 25 rue du Dr Roux, F75724 Paris cedex 15, France

^* To whom correspondence should be addressed. Email: s.j.w.busby{at}bham.ac.uk.

	Abstract

The Escherichia coli Rsd protein forms complexes with the RNA polymerase ⁷⁰ factor but its biological role is not understood. Transcriptome analysis shows that overexpression of Rsd causes increased expression from some promoters whose expression depends on the alternative ³⁸ factor, and this was confirmed by experiments with lac fusions at selected promoters. The LP18 substitution in Rsd increases the Rsd-dependent stimulation of these promoter::lac fusions. Analysis using a bacterial two hybrid system shows that the LP18 substitution in Rsd increases its interaction with ⁷⁰. Our experiments support a model in which the role of Rsd is primarily to sequester ⁷⁰, thereby increasing the levels of RNA polymerase containing the alternative ³⁸ factor.

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