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J. Bacteriol. doi:10.1128/JB.00084-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Identification of the L,D-Transpeptidases Responsible for Attachment of the Braun Lipoprotein to Escherichia coli Peptidoglycan

INSERM, U655-LRMA, Paris, F-75006 France, Université Pierre et Marie Curie-Paris6, Centre de Recherches Biomédicales des Cordeliers, Paris, F-75006 France, Université Paris-Descartes, Faculté de Médecine René Descartes, Centre de Recherches Biomédicales des Cordeliers, Paris, F-75006 France, AP-HP, Hôpital Européen Georges Pompidou, Paris, F-75015 France, Muséum National d'Histoire Naturelle, Plateforme de Spectrométrie de Masse et de Protéomique, Département Recherche Développement et Diversité Moléculaire, Paris, F-75005 France, Laboratoire des Enveloppes Bactériennes et Antibiotiques, UMR 8619 CNRS, Université Paris-Sud, 91405 Orsay, France

^* To whom correspondence should be addressed. Email: sophie.magnet{at}bhdc.jussieu.fr.

	Abstract

The Ldt_fm L,D-transpeptidase catalyzes peptidoglycan cross-linking in -lactam-resistant mutants of Enterococcus faecium. Here, we show that in Escherichia coli Ldt_fm homologues are responsible for attachment of the Braun lipoprotein to murein indicating that evolutionary-related domains have been tailored to use muropeptides or proteins as acyl acceptors in the L,D-transpeptidation reaction.

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