JB Accepts, published online ahead of print on 20 April 2007
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J. Bacteriol. doi:10.1128/JB.00109-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Structure-based functional analysis of plasmid R6K's replication protein: Key amino acids at the {pi}/DNA interface

Selvi Kunnimalaiyaan, Sheryl A. Rakowski, and Marcin Filutowicz*

Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison, Wisconsin 53706

* To whom correspondence should be addressed. Email: msfiluto{at}facstaff.wisc.edu.


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Abstract

In previous work, we characterized the bases in an iteron of plasmid R6K that are important for the binding of {pi} protein monomers and dimers. Here, we investigate six amino acids of {pi}, encoded by pir, hypothesized to be important for DNA contact: Ser71, Try74, Gly131, Gly211, Arg225 and Arg254.






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