Isolation of the rstA gene as a multicopy suppressor of YjeE, an essential ATPase of unknown function in Escherichia coli

Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5

^* To whom correspondence should be addressed. Email: ebrown{at}mcmaster.ca.

	Abstract

YjeE is an essential ATPase in Escherichia coli, whose cellular function remains uncharacterized. Using a genomic library we have identified rstA as a multicopy suppressor of a conditional yjeE deletion strain. High copy rstA is the first recorded suppressor for a lesion in yjeE and this newly charted genetic interaction has the potential to inform on the function with further study of the interacting partners.