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J. Bacteriol. doi:10.1128/JB.00193-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The role of RNA structure and susceptibility to RNase E in the regulation of a cold-shock mRNA, cspA mRNA

Department of Biochemistry & Molecular Biology, University of British Columbia, Life Sciences Centre, 2350 Health Sciences Mall, Vancouver BC, Canada, V6T 1Z3

^* To whom correspondence should be addressed. Email: gamackie{at}interchange.ubc.ca.

	Abstract

Degradation of the cspA mRNA in vivo is very rapid at temperatures greater than 30_ and is moderately dependent on RNase E. Investigations in vitro show that degradosomes prepared from normal or cold-shocked cultures cleave the cspA mRNA preferentially at a single site in vitro between two stem-loops 24 residues 3' to the termination codon and 31 residues from the 3'-end. The site of cleavage is independent of the temperature and largely independent of the phosphorylation status of the 5'-end of cspA mRNA. A 5'-stem-loop, potential occlusion of the initiation and termination codons, temperature-dependent translational efficiency and the position of the RNase E cleavage site can explain the differential stability of the cspA mRNA.