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J. Bacteriol. doi:10.1128/JB.00368-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Cell wide responses to low oxygen exposure in Desulfovibrio vulgaris Hildenborough

Virtual Institute of Microbial Stress and Survival; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, USA; Department of Chemical Engineering, University of California, Berkeley, USA; Department of Bioengineering, University of California, Berkeley, USA; Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, USA; Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA; Biochemistry and the Molecular Microbiology & Immunology Departments. University of Missouri, Columbia, USA; Institute for Environmental Genomics and Department of Botany and Microbiology, Oklahoma University, Norman, OK, USA

^* To whom correspondence should be addressed. Email: keasling{at}berkeley.edu.

	Abstract

The responses of the anaerobic, sulfate-reducing Desulfovibrio vulgaris Hildenborough to low oxygen exposure (0.1% O₂) were monitored via transcriptomics and proteomics. Exposure to 0.1% O₂ caused a decrease in growth rate without affecting viability. A concerted up-regulation in the predicted peroxide stress response regulon (PerR) genes was observed in response to the 0.1% O₂ exposure. Several of these candidates also showed increases in protein abundance. Among the remaining small number of transcript changes was the upregulation of the predicted transmembrane tetraheme cytochrome c₃ complex. Other known oxidative stress response candidates remained unchanged during this low O₂ exposure. To fully understand the results of the 0.1% O₂ exposure, transcriptomics and proteomics data were collected for exposure to air using a similar experimental protocol. In contrast to the 0.1% O₂ exposure, air exposure was detrimental to both the growth rate and viability and caused dramatic changes at both the transcriptome and proteome levels. Interestingly, the transcripts of the predicted PerR regulon genes were down regulated during air exposure. Our results highlight the differences in the cell wide response to low and high O₂ levels of in D. vulgaris and suggest that while exposure to air ishighly detrimental to D. vulgaris, this bacterium can successfully cope with periodic exposure to low O₂ levels in its environment.

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