The NtcA-regulated amtB gene is necessary for full methylammonium uptake activity in the cyanobacterium Synechococcus elongatus

Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, E-41092 Seville, Spain

^* To whom correspondence should be addressed. Email: eflores{at}ibvf.csic.es.

	Abstract

The Amt proteins constitute a ubiquitous family of transmembrane ammonia channels that permit the net uptake of ammonium by the cells. In many organisms, there is more than one amt gene, which are subjected to nitrogen control. The mature Amt protein is a homo- or hetero-oligomer of three Amt subunits. We have previously characterized an amt1 gene in the unicellular cyanobacterium Synechococcus elongatus strain PCC 7942. In this work, we describe the presence in this organism of a second amt gene, amtB, which encodes a protein more similar to the bacterial AmtB proteins than to any other cyanobacterial Amt. The expression of amtB took place in response to nitrogen step-down, required the NtcA transcription factor, and was parallel to that of amt1. However, transcript levels of amtB, measured after 2 h of nitrogen deprivation, were about 100-fold lower than those of amt1. A S. elongatus amtB insertional mutant exhibited an activity of uptake of [¹⁴C]methylammonium about 55% that observed in the wild type, but the inactivation of amtB had no noticeable effect on the uptake of ammonium when it was supplied at concentrations of 100 µM or above. Because the S. elongatus amt1 mutant is essentially devoid of [¹⁴C]methylammonium uptake activity, the mature Amt transporter is functional in the absence of AmtB subunits but not in the absence of Amt1 subunits. However, the S. elongatus amtB mutant could not concentrate [¹⁴C]methylammonium within the cells to the same extent as the wild type. Therefore, AmtB is necessary for full methylammonium uptake activity in S. elongatus.