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J. Bacteriol. doi:10.1128/JB.00472-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Genome Rearrangements, Deletions and Amplifications in the Natural Population of Bartonella henselae

Department of Molecular Evolution, Evolutionary Biology Center, Uppsala University, Uppsala, 752 36, Sweden; These authors contributed equally to this work

^* To whom correspondence should be addressed. Email: Siv.Andersson{at}ebc.uu.se.

	Abstract

Cats are the natural host for Bartonella henselae, an opportunistic human pathogen and the agent of cat-scratch disease. Here, we have analysed the natural variation in gene content and genome structure of 38 Bartonella henselae strains isolated from cats and humans by comparative genome hybridizations (CGH) to microarrays and probe hybridizations to pulse-field gel electrophoresis (PFGE) blots. The variation in gene content was modest and confined to the prophage and the genomic islands, whereas the PFGE analyses indicated extensive rearrangements across the terminus of replication with breakpoints in areas of the genomic islands. We observed no difference in gene content or structure between feline and human strains. Rather, the results suggest multiple sources of human infection from feline B. henselae strains of diverse genotypes. Additionally, the microarray hybridizations revealed DNA amplification in some strains in the so-called chromosome II-like region. The amplified segments were centred at a position corresponding to a putative phage replication initiation site and increased in size with the duration of cultivation. We hypothesize that the variable gene pool in the B. henselae population plays an important role in the establishment of long-term persistent infection in the natural host by promoting antigenic variation and escape from the host immune response.

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