This Article Full Text (PDF) Other Versions of this Article: JB.00491-07v1 189/17/6501    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Noriega, C. E. Articles by Rowe, J. J. Search for Related Content PubMed PubMed Citation Articles by Noriega, C. E. Articles by Rowe, J. J.

 Previous Article  |  Next Article 

J. Bacteriol. doi:10.1128/JB.00491-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Artificial control of Nitrate Respiration through the lac Promoter Permits the Assessment of Oxygen Mediated Posttranslational Regulation of the nar Operon in Pseudomonas aeruginosa

Department of Biology, University of Dayton, Dayton, OH 45469

^* To whom correspondence should be addressed. Email: John.Rowe{at}notes.udayton.edu.

	Abstract

In this study, oxygen and nitrate regulation of transcription and subsequent protein expression of the unique narK1K2GHJI respiratory operon of P. aeruginosa were investigated. Under the control of P_LAC, P. aeruginosa was able to transcribe nar, and subsequently express methyl viologen-linked nitrate reductase activity under aerobic conditions without nitrate. Modulation of P_LAC through the LacI repressor enabled us to assess both transcriptional and posttranslational regulation by oxygen during physiological whole cell nitrate reduction.