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J. Bacteriol. doi:10.1128/JB.00543-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

A Protein Modulator of Multidrug Efflux Gene Expression in Pseudomonas aeruginosa

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada, K7L 3N6, Infectious Diseases, Novartis Institute for Biomedical Research, Inc., 100 Technology Square, Cambridge, MA 02139, USA, Department of Biochemistry and Molecular Biology, University of British Columbia, 2146 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3

^* To whom correspondence should be addressed. Email: poolek{at}post.queensu.ca.

	Abstract

nalC multidrug-resistant mutants of P. aeruginosa show enhanced expression of the mexAB-oprM multidrug efflux system as a direct result of the production of a ca. 6,100 Dalton protein, PA3719, in these mutants. Using a bacterial 2-hybrid system, PA3719 was shown to interact in vivo with MexR, a repressor of mexAB-oprM expression. Isothermal titration calorimetry (ITC) studies confirmed a high affinity (K_D = 158.0 ± 18.1 nM) interaction of PA3719 with MexR in vitro. PA3719 binding to and formation of a complex with MexR obviated repressor binding to its operator, which overlaps the efflux operon promoter, suggesting that mexAB-oprM hyperexpression in nalC mutants results from PA3719 modulation of MexR repressor activity. Consistent with this, MexR repression of mexA transcription in an in vitro transcription assay was alleviated by PA3719. Mutations in MexR compromising its interaction with PA3719 in vivo were isolated and seen to be located internally and distributed throughout the protein, suggesting that they impacted PA3719 binding by altering MexR structure/conformation rather than residues interacting specifically with PA3719. Four of 6 mutant MexR proteins studied retained repressor activity even in a nalC strain producing PA3719. Again, this is consistent with a PA3719 interaction with MexR being necessary to obviate MexR repressor activity. The gene encoding PA3719 has, thus, been renamed armR (anti-repressor for MexR). A representative ‘non-interacting’ mutant MexR protein, MexR_I104F, was purified and ITC confirmed that it bound PA3719 with reduced affinity (5.4-fold; K_D = 853.2 ± 151.1 nM). Consistent with this, MexR_I104F repressor activity, as assessed using the in vitro transcription assay, was only weakly compromised by PA3719. Finally, 2 mutations (L36P and W45A) in ArmR compromising its interaction with MexR have been isolated and mapped to a putative C-terminal -helix of the protein that alone is sufficient for interaction with MexR.