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J. Bacteriol. doi:10.1128/JB.00564-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The intracellular concentration of acetyl phosphate in Escherichia coli is sufficient for direct phosphorylation of two-component response regulators

Department of Microbiology and Immunology, Loyola University Chicago, 2160 S. First Ave. Bldg. 105, Maywood, IL 60153

^* To whom correspondence should be addressed. Email: awolfe{at}lumc.edu.

	Abstract

Acetyl phosphate, the intermediate of the AckA-Pta pathway, acts as a global signal in Escherichia coli. Although acetyl phosphate clearly signals through two-component response regulators, it remains unclear whether acetyl-P acts as a direct phospho-donor or functions through an indirect mechanism. We have used two-dimensional thin layer chromatography to measure the relative concentrations of acetyl phosphate, acetyl-coenzyme A, ATP and GTP over the course of the entire growth curve. We have estimated the intracellular concentration of acetyl phosphate in wild-type cells to reach at least 3 mM, a concentration sufficient to activate two-component response regulators via direct phosphoryl transfer.

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