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J. Bacteriol. doi:10.1128/JB.00584-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Proteomics and Transcriptomics Analysis of Extracellular Proteins and mRNA Levels in Thermobifida fusca Grown on Cellobiose and Glucose

Department of Molecular Biology and Genetics, 458 Biotechnology Building, Cornell University, Ithaca, NY 14853

^* To whom correspondence should be addressed. Email: dbw3{at}cornell.edu.

	Abstract

Thermobifida fusca secretes proteins that carry out plant cell wall degradation. Using two-dimensional electrophoresis the extracellular proteome of T. fusca grown on cellobiose was compared with that from glucose grown cells. Extracellular proteins whose expression is induced by cellobiose are mainly cellulases and cellulose-binding proteins. Other major extracellular proteins induced by cellobiose include a xylanase (Xyl10A) and two unknown proteins whose C-terminal regions are homologous to a Lytic Transglycosylase/Goose Egg White Lysozyme (LT_GEWL) domain and a NLPC_P60 domain, respectively. Transcriptional analysis of genes encoding cellobiose-induced proteins suggests that their expression is controlled at the transcriptional level and that their expression is also induced by cellulose. Some other major extracellular proteins produced by T. fusca grown on both cellobiose and glucose include Lam81A and three unknown proteins that are homologous to aminopeptidases, xylanases or contain a putative NLPC_P60 domain, respectively.