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J. Bacteriol. doi:10.1128/JB.00667-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

"Transcriptional Responses of Mycoplasma gallisepticum Strain R In Association with Eukaryotic Cells"

Center of Excellence for Vaccine Research, Department of Pathobiology and Veterinary Sciences, University of Connecticut

^* To whom correspondence should be addressed. Email: steven.geary{at}uconn.edu.

	Abstract

Mycoplasma gallisepticum is an etiologic agent of chronic respiratory disease (CRD) in chickens and infectious sinusitis in turkeys. Other than proteins important for cytadherence, few M. gallisepticum factors or pathways contributing to host cell interactions have been identified. In this study, an oligonucleotide-based microarray was utilized to investigate transcriptional changes in M. gallisepticum strain R_low upon exposure to eukaryotic cells. Fifty-eight genes were either up- or down-regulated upon exposure to MRC-5 lung fibroblasts grown in vitro, including genes encoding transport-, metabolism-, and translation-associated proteins. Twenty of the 58 regulated genes have no assigned function. These results indicate that M. gallisepticum regulates gene expression upon exposure to eukaryotic cells, revealing genes and pathways likely to be important for host-bacterial interaction.