This Article Full Text (PDF) Other Versions of this Article: JB.00671-06v1 188/21/7600    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shah, R. Articles by Emerich, D. W. Search for Related Content PubMed PubMed Citation Articles by Shah, R. Articles by Emerich, D. W.

 Previous Article  |  Next Article 

J. Bacteriol. doi:10.1128/JB.00671-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Isocitrate Dehydrogenase of Bradyrhizobium japonicum is not required for symbiotic nitrogen fixation with soybean

Department of Biochemistry, University of Missouri, Columbia, Missouri, 65211; Qiagen, Inc. 28159 Avenue Stanford, Valencia CA, 91355

	Abstract

A mutant strain of Bradyrhizobium japonicum USDA110 lacking isocitrate dehydrogenase activity was created to determine whether this enzyme was required for symbiotic nitrogen fixation with soybean (Glycine max cv. Williams 82). The isocitrate dehydrogenase mutant, strain 5051, was constructed by insertion of a streptomycin resistance gene cassette. The mutant was devoid of isocitrate dehydrogenase activity and of immunologically-detectable protein indicating there is only one copy in the genome. Strain 5051 grew well on a variety of carbon sources including arabinose, pyruvate, succinate and malate, but unlike many microorganisms, was a glutamate auxotroph. Although the formation of nodules were slightly delayed, the mutant was able to form nodules on soybean and reduce atmospheric dinitrogen as well as the wild type indicating that the plant was able to supply sufficient glutamate to permit infection. Combined with the results of other citric acid cycle mutants, these results suggest a role for the citric acid cyle in the infection and colonization stage of nodule development but not in the actual fixation of atmospheric dinitrogen.

This article has been cited by other articles:

• White, J., Prell, J., James, E. K., Poole, P. (2007). Nutrient Sharing between Symbionts. Plant Physiol. 144: 604-614 [Full Text]