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JB Accepts, published online ahead of print on 29 June 2007
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J. Bacteriol. doi:10.1128/JB.00687-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Identification of the Major Protein Component of the Pneumococcal Eclipse Complex

Donald A. Morrison*, Isabelle Mortier-Barrière, Laetitia Attaiech, and Jean-Pierre Claverys

Laboratoire de Microbiologie et Génétique Moléculaires, UMR 5100 CNRS-Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 9, France; Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, 900 South Ashland Ave., Chicago, IL 60607 USA


  Abstract

During genetic transformation of Streptococcus. pneumoniae, single strands from native donor DNA enter competent cells, where they associate with an unidentified protein of MW 15-20 kD to form the eclipse complex. Using Western blotting, we identify the principal protein co-fractionating with donor DNA in this complex as SsbB.




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