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J. Bacteriol. doi:10.1128/JB.00738-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Transducing Particles of Staphylococcus aureus Pathogenicity Island SaPI1 are Comprised of Helper Phage-Encoded Proteins

Departments of Microbiology and Immunology, and Pharmacology and Toxicology, Virginia Commonwealth University School of Medicine

^* To whom correspondence should be addressed. Email: christie{at}vcu.edu.

	Abstract

The relationship between the composition of SaPI1 transducing particles and those of helper phage 80 was investigated by direct comparison of virion proteins. Twelve virion proteins were identified by SDS-PAGE and mass spectrometry; all were present in both 80 and SaPI1 virions and all were encoded by 80. No SaPI1-encoded proteins were detected. This confirms the prediction that SaPI1 is encapsidated in a virion assembled from helper phage-encoded proteins.

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