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J. Bacteriol. doi:10.1128/JB.00801-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Neisseria gonorrhoeae DNA recombination and repair enzymes protect against oxidative damage caused by hydrogen peroxide

^* To whom correspondence should be addressed. Email: e-stohl{at}northwestern.edu,

	Abstract

The strict human pathogen Neisseria gonorrhoeae (Gc) is exposed to oxidative damage during infection. Gc has many defenses that have been demonstrated to counteract oxidative damage. However, recN is the only DNA repair and recombination enzyme upregulated in response to hydrogen peroxide (H₂O₂) by microarray analysis and subsequently shown to be important for oxidative damage protection. We therefore tested the importance of RecA and DNA recombination and repair enzymes in conferring resistance to H₂O₂ damage. recA mutants, as well as RecBCD (recB, recC, recD) and RecF-like pathway mutants (recJ, recO, and recQ), all showed decreased resistance to H₂O₂. Holliday junction processing mutants (ruvA, ruvC, recG) showed decreased resistance to H₂O₂ resistance as well. Finally, we showed that RecA protein levels did not increase as a result of H₂O₂ treatment. We propose that RecA, recombinational DNA repair, and branch migration are all important for H₂O₂ resistance in Gc, but that constitutive levels of these enzymes are sufficient for providing protection against oxidative damage by H₂O₂.

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