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J. Bacteriol. doi:10.1128/JB.00821-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Sigma factor genes sigC, sigE, and sigG are upregulated in heterocysts of the cyanobacterium Anabaena sp. strain PCC 7120

Department of Biology, Texas A&M University, College Station, Texas

^* To whom correspondence should be addressed. Email: jgolden{at}tamu.edu.

	Abstract

We used gfp transcriptional fusions to investigate the regulation of eight sigma factor genes during heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120. Reporter strains containing gfp fusions with the upstream regions of sigB2, sigD, sigI, and sigJ did not show developmental regulation. Time-lapse microscopy of sigC, sigE, and sigG reporter strains showed increased GFP fluorescence in differentiating cells at 4 h, 16 h, and 9 h, respectively, after nitrogen step-down.

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• Valladares, A., Flores, E., Herrero, A. (2008). Transcription Activation by NtcA and 2-Oxoglutarate of Three Genes Involved in Heterocyst Differentiation in the Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 190: 6126-6133 [Abstract] [Full Text]