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J. Bacteriol. doi:10.1128/JB.00859-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Interaction of related Tn916-like transposons: analysis of excision events promoted by Tn916 and Tn5386 integrases

From the Medicine and Research Services, Louis Stokes Cleveland VA Medical Center and the Department of Medicine, Case Western Reserve University, Cleveland, Ohio

^* To whom correspondence should be addressed. Email: louis.rice{at}med.va.gov.

	Abstract

In recent work, we described excision of a large genomic region from Enterococcus faecium D344R in which sequence from "joint" regions suggested that excision resulted from the interaction of conjugative transposon Tn916 and the related mobile element Tn5386. In the present study, we examined the ability of integrases and integrase/excisase combinations from Tn916 and Tn5386 to promote excision of constructs consisting of the termini of Tn916, Tn5386 and the VanB mobile element Tn5382. Integrases alone from either Tn916 or Tn5386 promoted circularization of constructs from the three different transposons, even when the different termini used in the constructs were discordant in their transposon of origin. The termini of Tn916 and Tn5382 found in all joints were consistent with previously identified Tn916 and Tn5382 termini. Substantial variation was seen in the integrase terminus of Tn5386 used to form joints, regardless of the integrase that was responsible for circularization. Variability was observed in joints formed from Tn5386 constructs, in contrast to joints observed with the termini of Tn916 or Tn5382. The co-expression of excisase yielded some variability in the joint regions observed. These data confirm that integrases from some Tn916-like elements can promote circularization with termini derived from heterologous transposons and as such could promote excision of large genomic regions flanked by such elements. They also raise interesting questions about sequence specificities of the C-terminals of Tn916-like integrases, which bind to the ends and facilitate strand exchange.