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J. Bacteriol. doi:10.1128/JB.00889-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Transcriptomic analysis of the sulfate starvation response of Pseudomonas aeruginosa

Faculty of Life Sciences, University of Manchester, Oxford Rd, Manchester, M13 9PT, UK, Génétique Moléculaire, Génomique, Microbiologie, UMR 7156 Université Louis Pasteur - CNRS, F-67083 Strasbourg, France, and Faculty of Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom

^* To whom correspondence should be addressed. Email: michael.kertesz{at}manchester.ac.uk.

	Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that causes a number of infections in humans, but is best known for its association with cystic fibrosis. It is able to use a wide range of sulfur compounds as sources of sulfur for growth. Gene expression in response to changes in sulfur supply was studied in P. aeruginosa E601, a cystic fibrosis isolate that displays mucin sulfatase activity, and in P. aeruginosa PAO1. A large family of genes was found to be upregulated by sulfate limitation in both isolates, encoding sulfatases and sulfonatases, transport systems, oxidative stress proteins, and a sulfate-regulated TonB/ExbBD complex. These genes were localized in five distinct islands on the genome, and encoded proteins with a significantly reduced content of cysteine and methionine. Growth of P. aeruginosa E601 with mucin as sulfur source led to a sulfate starvation response, but also to induction of genes involved with type III secretion systems.