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J. Bacteriol. doi:10.1128/JB.00899-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The Polymerization of Actin-Like Protein MamK Associated with Magnetosomes

Department of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan, and Laboratoire de Chimie Bactérienne, UPR9043, IBSM, CNRS, 31, chemin Joseph Aiguier, 13402 Marseille cedex 20, France

^* To whom correspondence should be addressed. Email: fukumor{at}kenroku.kanazawa-u.ac.jp.

	Abstract

The recombinant actin-like protein MamK was purified from Escherichia coli, and used as an antigen to generate the anti-MamK antibody. Immunostaining studies showed a linear distribution of MamK in Magnetospirillum magnetotacticum cell, and MamK in association with magnetosomes. Moreover, we demonstrated that MamK polymerizes into filamentous bundle in vitro.