JB Accepts, published online ahead of print on 10 August 2007
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J. Bacteriol. doi:10.1128/JB.00968-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Complex formation of the mrp(sha)ABCDEFG gene products that constitute a principle Na+/H+ antiporter in Bacillus subtilis

Yusuke Kajiyama, Masato Otagiri, Junichi Sekiguchi, Saori Kosono*, and Toshiaki Kudo

Environmental Molecular Biology Laboratory, RIKEN, Department of Applied Biology, Shinshu University, Graduate School of Integrated Science, Yokohama City University, Japan

* To whom correspondence should be addressed. Email: kosono{at}riken.jp.


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Abstract

The Bacillus subtilis Mrp (also referred to as Sha) is a particularly unique Na+/H+ antiporter encoded by mrpABCDEFG. Using His-tagging of Mrp proteins, we showed complex formation of the mrpABCDEFG gene products by pull-down and blue native PAGE analyses. This is the first molecular evidence that the Mrp is a multi-component antiporter in the cation-proton antiporter-3 family.




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