JB Accepts, published online ahead of print on 1 September 2006

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ross, C. Articles by Robleto, E. Search for Related Content PubMed PubMed Citation Articles by Ross, C. Articles by Robleto, E.

 Previous Article  |  Next Article 

J. Bacteriol. doi:10.1128/JB.00980-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Novel role of mfd: Effects on Stationary Phase Mutagenesis in Bacillus subtilis

Christian Ross, Christine Pybus, Mario Pedraza-Reyes, Huang-Mo Sung, Ronald E. Yasbin, and Eduardo Robleto^*

Department of Biological Sciences, University of Nevada Las Vegas, Las Vegas, Nevada, 89154-4004; Institute of Investigation in Experimental Biology, Faculty of Chemistry, University of Guanajuato, Guanajuato, Mexico, 36050

^* To whom correspondence should be addressed. Email: eduardo.robletoe{at}unlv.edu.

Previously, we established, in a chromosomal reversion assay system, that an adaptive mutagenic process occurs in non-growing Bacillus subtilis cells under stress and demonstrated that multiple mechanisms are involved in generating these mutations . In an attempt to delineate how these mutations are generated, we began an investigation into whether or not transcription and transcription-associated proteins influence adaptive mutagenesis. In B. subtilis, the Mfd protein (transcription repair coupling factor, or TRCF) facilitates removal of RNA polymerase stalled at transcriptional blockages and recruitment of repair proteins to DNA lesions on the transcribed strand. Here we demonstrate that the loss of Mfd has a depressive effect on stationary phase mutagenesis. An association between Mfd mutagenesis and aspects of transcription is discussed.

This article has been cited by other articles:

• Tsai, Y.-K., Chen, H.-W., Lo, T.-C., Lin, T.-H. (2009). Specific point mutations in Lactobacillus casei ATCC 27139 cause a phenotype switch from Lac- to Lac+. Microbiology 155: 751-760 [Abstract] [Full Text]  
• Vidales, L. E., Cardenas, L. C., Robleto, E., Yasbin, R. E., Pedraza-Reyes, M. (2009). Defects in the Error Prevention Oxidized Guanine System Potentiate Stationary-Phase Mutagenesis in Bacillus subtilis. J. Bacteriol. 191: 506-513 [Abstract] [Full Text]  
• Hasegawa, K., Yoshiyama, K., Maki, H. (2008). Spontaneous mutagenesis associated with nucleotide excision repair in Escherichia coli.. GENES CELLS 13: 459-469 [Abstract] [Full Text]