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J. Bacteriol. doi:10.1128/JB.01011-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Surface Protein IsdC and Sortase B are Required for Heme-Iron Scavenging of Bacillus anthracis

Department of Microbiology, University of Chicago, 920 E 58^th Street, Chicago, Illinois 60637, USA

^* To whom correspondence should be addressed. Email: oschnee{at}bsd.uchicago.edu.

	Abstract

Bacillus anthracis, the spore-forming agent of anthrax, requires iron for growth and is capable of scavenging heme-iron during infection. We show here that the B. anthracis iron-regulated surface determinants (isd) locus encompasses isdC, specifying a heme-iron binding surface protein. Anchoring of IsdC to the cell wall envelope of vegetative bacilli requires srtB, which encodes sortase B. Purified sortase B cleaves IsdC between the threonine and the glycine of its NPKTG motif sorting signal. B. anthracis variants lacking either isdC or srtB display defects in heme-iron scavenging, suggesting that IsdC binding to heme-iron in the cell wall envelope contributes to bacterial uptake of heme.

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