Identification of promoters recognized by RNA polymerase-^E holoenzyme from Thermus thermophilus HB8

RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan; RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan; Genomic Sciences Center, Yokohama Institute, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan; Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan; and Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

^* To whom correspondence should be addressed. Email: ashinkai{at}spring8.or.jp. yokoyama{at}biochem.s.u-tokyo.ac.jp.

	Abstract

T. thermophilus ^E, an extracytoplasmic function factor from extremely thermophilic bacterium Thermus thermophilus HB8, bound to the RNA polymerase core enzyme and showed transcriptional activity. With the combination of in vitro transcription assaying and GeneChip technology, we identified three promoters recognized by ^E. The predicted consensus promoter sequence for ^E is 5'-CA(A/T)(A/C)C(A/C)-N₁₅-CCGTA-3'.