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J. Bacteriol. doi:10.1128/JB.01079-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The sbcDC locus mediates the repression of type 5 capsule as part of the SOS response in Staphylococcus aureus

Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72205

^* To whom correspondence should be addressed. Email: clee2{at}uams.edu.

	Abstract

Most strains of Staphylococcus aureus produce one type of capsular polysaccharide that belongs to either type 5 or type 8. The production of these capsules has been shown to be regulated by various regulators. Here we report that the sbcD and sbcC genes are involved in the repression of type 5 capsule production. Chromosomal deletions in the sbcDC genes resulted in increased capsule promoter activity, capsule gene transcripts and capsule production. The survival rates of the sbcDC deletion mutant were reduced upon UV irradiation compared to the wild type strain Newman suggesting that the genes are involved in DNA repair in S. aureus. The two genes were organized as an operon and were expressed very early in the exponential growth phase. Subinhibitory concentration of ciproflaxocin or mitomycin C induced sbcDC transcription but repressed the capsule promoter activity suggesting that the sbcDC genes and the capsule genes are part of the SOS regulon. By reporter gene fusion and Northern blotting, we found that sbcDC regulated capsule by downregulating arl and mgr. Further genetic studies indicate that sbcDC functions upstream of the arl and mgr in capsule regulation. Collectively, our results indicate that sbcDC, upon SOS response, represses type 5 capsule production through arl-mgr pathway. To our knowledge, this is the first demonstration that a SbcDC homolog was involved in transcriptional regulation.