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J. Bacteriol. doi:10.1128/JB.01138-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Nutritional cues control Pseudomonas aeruginosa multi-cellular behavior in cystic fibrosis sputum

Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, TX

^* To whom correspondence should be addressed. Email: mwhiteley{at}mail.utexas.edu.

	Abstract

The sputum (mucus) layer of the cystic fibrosis (CF) lung is a complex substrate that provides Pseudomonas aeruginosa with carbon and energy to support high density growth during chronic colonization. Unfortunately, the CF lung sputum layer has been difficult to mimic in animal models of CF disease, and mechanistic studies of P. aeruginosa physiology during growth in CF sputum are hampered by its complexity. In this study, we performed chromatographic and enzymatic analyses of CF sputum to develop a defined, Synthetic CF sputum Medium (SCFM) that mimics the nutritional composition of CF sputum. Importantly, P. aeruginosa displays similar phenotypes during growth in CF sputum and SCFM including similar growth rates, gene expression profiles, carbon substrate preference, and cell-cell signaling profiles. Using SCFM, we provide evidence that aromatic amino acids serve as nutritional cues that influence cell-cell signaling and antimicrobial activity of P. aeruginosa during growth in CF sputum.

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