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J. Bacteriol. doi:10.1128/JB.01215-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

AMINO ACID TOXICITIES OF ESCHERICHIA COLI THAT ARE PREVENTED BY LEUCYL-tRNA SYNTHETASE AMINO ACID EDITING

Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave. 419 Roger Adams Laboratory, Box B-4, Urbana, IL 61801-3732

^* To whom correspondence should be addressed. Email: martinis{at}life.uiuc.edu.

	Abstract

Leucyl-tRNA synthetase (LeuRS) has evolved an editing function to clear misactivated amino acids. An Escherichia coli-based assay was established to identify amino acids that compromise the fidelity of LeuRS and translation. Multiple non-standard, as well as standard amino acids were toxic to the cell when LeuRS editing was inactivated.

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