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J. Bacteriol. doi:10.1128/JB.01217-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Trans-acting factors and cis-elements involved in Glucose Repression of Arabinan Degradation in Bacillus subtilis

Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República, Apartado 127, 2781-901 Oeiras, Portugal; Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Quinta da Torre, 2829-516 Caparica, Portugal

^* To whom correspondence should be addressed. Email: sanoguei{at}itqb.unl.pt.

	Abstract

In Bacillus subtilis the synthesis of enzymes involved in the degradation of arabinose-containing polysaccharides, is subjected to carbon catabolite repression. Here, we show that CcpA is the major regulator of repression of the arabinases genes in the presence of glucose. CcpA acts via binding to one cre element in each promoter region of the abnA and xsa genes and to two cres in the araABDLMNPQ-abfA operon. Contributions of co-effectors HPr and Crh to CCR differ according to growth phase. HPr dependency occurs both during exponential growth and transition phase, while Crh dependency is mainly detected at transition phase. Our results suggest that Crh synthesis may increase at the end of exponential growth and consequently contribute to this effect together with other factors.

This article has been cited by other articles:

• Inacio, J. M., Correia, I. L., de Sa-Nogueira, I. (2008). Two distinct arabinofuranosidases contribute to arabino-oligosaccharide degradation in Bacillus subtilis. Microbiology 154: 2719-2729 [Abstract] [Full Text]  
• Inacio, J. M., de Sa-Nogueira, I. (2008). Characterization of abn2 (yxiA), Encoding a Bacillus subtilis GH43 Arabinanase, Abn2, and Its Role in Arabino-Polysaccharide Degradation. J. Bacteriol. 190: 4272-4280 [Abstract] [Full Text]