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J. Bacteriol. doi:10.1128/JB.01321-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Mutation at different sites in the Nostoc punctiforme cyaC gene, encoding the multiple domain enzyme adenylate cyclase, results in different levels of infection of the host plant Blasia pusilla.

Institute of Integrative and Comparative Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, United Kingdom

^* To whom correspondence should be addressed. Email: d.g.adams{at}leeds.ac.uk.

	Abstract

The filamentous cyanobacterium Nostoc punctiforme forms symbioses with plants. Disruption of the catalytic domain of the N. punctiforme adenylate cyclase (CyaC) significantly increased symbiotic competency, whereas reduced infectivity was observed in a mutant disrupted close to the N-terminus of CyaC. Total cellular cAMP levels were significantly reduced in both mutants.