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J. Bacteriol. doi:10.1128/JB.01368-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Escherichia coli Physiology in Luria-Bertani Broth

Institut Jacques Monod (C.N.R.S., Université Paris 6, Université Paris 7), F-75251 Paris Cedex 05, France

^* To whom correspondence should be addressed. Email: sezonov{at}pasteur.fr.

	Abstract

Luria-Bertani broth supports Escherichia coli growth to OD₆₀₀ 7. Surprisingly, however, steady state growth ceases at OD₆₀₀ 0.3, when the growth rate slows down and cell mass decreases. Growth stops for lack of a utilisable carbon source. Carbon sources for E. coli in Luria-Bertani broth are catabolyzable amino acids, not sugars.