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J. Bacteriol. doi:10.1128/JB.01402-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The broad host range Yersinia phage PY100: genome sequence, proteome analysis of virions and DNA packaging strategy

Bundesinstitut für Risikobewertung, Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany; Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden; Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany

^* To whom correspondence should be addressed. Email: Eckhard.Strauch{at}bfr.bund.de.

	Abstract

PY100 is a lytic bacteriophage with a broad host range within the genus Yersinia. The phage forms plaques on strains of the three human pathogenic species Y. enterocolitica, Y. pseudotuberculosis and Y. pestis at 37°C. PY100 was isolated from farm manure and intended to be used in phage therapy trials. PY100 has an icosahedral capsid containing double stranded DNA and a contractile tail. The genome consists of 50,291 bp and is predicted to contain 93 open reading frames. Homology of PY100 gene products were found to the capsid proteins and proteins involved in DNA metabolism of the enterobacterial phage T1; PY100 tail proteins possess homologies to putative tail proteins of phage aa23 of Actinobacillus actinomycetemcomitans. In a proteome analysis of virion particles 15 proteins of the head and tail structures were identified by mass spectrometry. The putative gene product of ORF2 of PY100 shows significant homology to the gene 3 product (small terminase subunit) of Salmonella phage P22 which is involved in packaging of the concatemeric phage DNA. The packaging mechanism of PY100 was analysed by hybridization and sequence analysis of DNA isolated from virion particles. Newly replicated PY100 DNA is cut initially at a pac recognition site, which is located in the coding region of ORF2.