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J. Bacteriol. doi:10.1128/JB.01444-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Enhanced Post-Stationary Phase Survival of a Clinical Thymidine-Dependent Small Colony Variant of Staphylococcus aureus Results From Lack of Functional TCA Cycle

Indranil Chatterjee*, Mathias Herrmann, Richard A. Proctor, Georg Peters, and Barbara C. Kahl

Institute of Medical Microbiology and Hygiene, Institutes of Infectious Disease Medicine, University of Saarland, Homburg/Saar, Germany; Department of Medicine and Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, Wisconsin, USA; Institute of Medical Microbiology, University Hospital of Münster, Münster, Germany

* To whom correspondence should be addressed. Email: ichatterjee76{at}yahoo.com.


   Abstract

The mechanisms of Staphylococcus aureus small colony variant (SCV) persistence are not fully elucidated. In this study, clinical thymidine-dependent SCVs displayed altered expression of citB, clpC and arcA, reduced acetate catabolization, and enhanced survival. These results implicate the importance of changes in tricarboxylic acid cycle and acetic acid metabolism in SCV survival and persistence.




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