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J. Bacteriol. doi:10.1128/JB.01462-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The "Cold-Sensitive" Mutants of Lac Repressor

Institut für Genetik der Universität zu Köln, Zülpicherstr. 47, D-50674 Köln, Germany; Western Australian Institute for Medical Research, Rear 50 Murray Street, Perth WA 6000, Australia; and IMBB-FORTH, Vassilika Vouton, P.O. Box 1378, GR-71110 Heraklion, Crete, Greece

^* To whom correspondence should be addressed. Email: oehler{at}imbb.forth.gr.

	Abstract

Thirteen of the more than 4,000 single amino acid replacements of Lac repressor, generated by suppression of amber nonsense mutants, were characterised as having a cold-sensitive phenotype. However, when expressed as missense mutations, none of the replacements causes cold-sensitivity, implicating the suppression mechanism as responsible for this phenotype.