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JB Accepts, published online ahead of print on 4 January 2008
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J. Bacteriol. doi:10.1128/JB.01524-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

DNA Microarray Analysis of the Central Carbohydrate Metabolism: Glycolytic/Gluconeogenic Carbon Switch in the Hyperthermophilic Crenarchaeum Thermoproteus tenax

Melanie Zaparty*, Alexander Zaigler, Claudia Stamme, Jörg Soppa, Reinhard Hensel, and Bettina Siebers

Department of Biology and Geography, Institute of Biology, Microbiology I, University of Duisburg-Essen, Universitätsstr. 5, 45117 Essen, Germany; Biocenter Niederursel, Institute of Microbiology, Goethe-University, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany

* To whom correspondence should be addressed. Email: melanie.zaparty{at}uni-due.de.


  Abstract

In order to unravel the role of regulation on transcript level in the central carbohydrate metabolism (CCM) of Thermoproteus tenax, a focused DNA microarray was constructed by using 85 open reading frames involved in the CCM. Transcriptional analysis was performed comparing heterotrophic growth on glucose versus autotrophic growth on CO2/H2.




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