This Article Full Text (PDF) Other Versions of this Article: JB.01586-06v1 189/5/1711    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Berkner, S. Articles by Lipps, G. Search for Related Content PubMed PubMed Citation Articles by Berkner, S. Articles by Lipps, G.

 Previous Article  |  Next Article 

J. Bacteriol. doi:10.1128/JB.01586-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Characterization of the transcriptional activity of the cryptic plasmid pRN1 from Sulfolobus islandicus REN1H1 and regulation of its replication operon

Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany

^* To whom correspondence should be addressed. Email: georg.lipps{at}uni-bayreuth.de.

	Abstract

The plasmid pRN1 from Sulfolobus islandicus REN1H1 belongs to the crenarchaeal plasmid family pRN. These plasmids encode three conserved proteins that participate in the plasmid replication and copy number regulation as suggested by biochemical characterization of the recombinant proteins. In order to deepen our understanding of the molecular biology of these plasmids we investigated the transcriptional activity of the model plasmid pRN1. We could detect five major transcripts present at about 2-15 copies per cell. One long transcriptional unit comprises the genes of the plasmid copy number control protein ORF56/CopG and of the replication protein ORF904. A second transcript with a long 3'-untranslated region codes for the DNA-binding protein ORF80. For both transcripts we could identify countertranscripts which could play a regulatory role. The function of the fifth transcript is unclear. For the five transcripts we determined the start site, the transcript end, the stability and the abundance in different growth phases. Reporter gene experiments demonstrate that the copy number control protein ORF56 represses transcription of the orf56/orf904 cotranscript in vivo.

This article has been cited by other articles:

• Contursi, P., Cannio, R., Prato, S., She, Q., Rossi, M., Bartolucci, S. (2007). Transcriptional Analysis of the Genetic Element pSSVx: Differential and Temporal Regulation of Gene Expression Reveals Correlation between Transcription and Replication. J. Bacteriol. 189: 6339-6350 [Abstract] [Full Text]  
• Berkner, S., Grogan, D., Albers, S.-V., Lipps, G. (2007). Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaea. Nucleic Acids Res 0: gkm449v1-12 [Abstract] [Full Text]  
• Geslin, C., Gaillard, M., Flament, D., Rouault, K., Le Romancer, M., Prieur, D., Erauso, G. (2007). Analysis of the First Genome of a Hyperthermophilic Marine Virus-Like Particle, PAV1, Isolated from Pyrococcus abyssi. J. Bacteriol. 189: 4510-4519 [Abstract] [Full Text]