This Article Full Text (PDF) Other Versions of this Article: JB.01623-06v1 189/5/2164    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Overhage, J. Articles by Hancock, R. E. W. Search for Related Content PubMed PubMed Citation Articles by Overhage, J. Articles by Hancock, R. E. W.

 Previous Article  |  Next Article 

J. Bacteriol. doi:10.1128/JB.01623-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Identification of genes involved in swarming motility using a Pseudomonas aeruginosa PAO1 mini-Tn5-lux mutant library

Centre for Microbial Diseases & Immunity Research, University of British Columbia, 2259 Lower Mall, Vancouver BC, Canada

^* To whom correspondence should be addressed. Email: bob{at}cmdr.ubc.

	Abstract

During a screening of a mini-Tn5-luxCDABE transposon mutant library of P. aeruginosa PAO1 for alterations in swarming motility, 36 mutants were identified with Tn5-insertions in the synthesis or function of flagellin and type IV pilus, in the Xcp-related type II secretion system, and in regulatory, metabolic, chemosensory, and hypothetical genes with unknown functions. These mutants were differentially affected in swimming and twitching motility but in most cases had only a minor additional motility defect. Our data provide evidence that swarming is a more complex type of motility, since it is influenced by a large number of different genes in P. aeruginosa. Conversely, many of the swarming-negative mutants showed also an impairment in biofilm formation indicating a strong relationship between these types of growth states.

This article has been cited by other articles:

• Overhage, J., Campisano, A., Bains, M., Torfs, E. C. W., Rehm, B. H. A., Hancock, R. E. W. (2008). Human Host Defense Peptide LL-37 Prevents Bacterial Biofilm Formation. Infect. Immun. 76: 4176-4182 [Abstract] [Full Text]  
• Gooderham, W. J., Bains, M., McPhee, J. B., Wiegand, I., Hancock, R. E. W. (2008). Induction by Cationic Antimicrobial Peptides and Involvement in Intrinsic Polymyxin and Antimicrobial Peptide Resistance, Biofilm Formation, and Swarming Motility of PsrA in Pseudomonas aeruginosa. J. Bacteriol. 190: 5624-5634 [Abstract] [Full Text]  
• Murray, T. S., Kazmierczak, B. I. (2008). Pseudomonas aeruginosa Exhibits Sliding Motility in the Absence of Type IV Pili and Flagella. J. Bacteriol. 190: 2700-2708 [Abstract] [Full Text]  
• Kukavica-Ibrulj, I., Bragonzi, A., Paroni, M., Winstanley, C., Sanschagrin, F., O'Toole, G. A., Levesque, R. C. (2008). In Vivo Growth of Pseudomonas aeruginosa Strains PAO1 and PA14 and the Hypervirulent Strain LESB58 in a Rat Model of Chronic Lung Infection. J. Bacteriol. 190: 2804-2813 [Abstract] [Full Text]  
• Goldberg, J. B., Hancock, R. E. W., Parales, R. E., Loper, J., Cornelis, P. (2008). Pseudomonas 2007. J. Bacteriol. 190: 2649-2662 [Full Text]  
• Overhage, J., Bains, M., Brazas, M. D., Hancock, R. E. W. (2008). Swarming of Pseudomonas aeruginosa Is a Complex Adaptation Leading to Increased Production of Virulence Factors and Antibiotic Resistance. J. Bacteriol. 190: 2671-2679 [Abstract] [Full Text]