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J. Bacteriol. doi:10.1128/JB.01669-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Evidence for Type III Restriction and Modification Systems in Mycoplasma pulmonis

Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294

^* To whom correspondence should be addressed. Email: dybvig{at}uab.edu.

	Abstract

Mycoplasma pulmonis possesses a cassette of genes that are predicted to code for type III restriction and modification (R-M) enzymes. Transposon disruption of a gene predicted to code for the endonuclease subunit of the enzyme resulted in loss of R-M activity. Genomic data indicate that the cassette was acquired by horizontal gene transfer and possibly located on a mobile element.