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J. Bacteriol. doi:10.1128/JB.01736-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Analysis of temporal gene expression during Bacillus subtilis spore germination and outgrowth

Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands; Microarray Department, University of Amsterdam, Amsterdam, The Netherlands; Unilever Food & Health Research Institute, Advanced Food Microbiology, Vlaardingen, The Netherlands; TNO Quality of Life, Food and Biotechnology Innovations - Microbiology, Zeist, The Netherlands

^* To whom correspondence should be addressed. Email: B.keijser{at}TNO.nl.

	Abstract

Bacillus subtilis forms dormant spores upon nutrient depletion. Under favorable environmental conditions, the spore breaks its dormancy and resumes growth in a process called spore germination and outgrowth. To elucidate the physiological processes that occur during the transition of the dormant spore to an actively growing vegetative cell, we studied this process in a time-dependent manner by a combination of microscopy, analysis of extracellular metabolites and a genome-wide analysis of transcription. The results indicate the presence of abundant levels of late sporulation transcripts in dormant spores. In addition, results suggest the existence of a complex and well-regulated spore outgrowth program, involving the temporal expression of at least 30 % of the B. subtilis genome.

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