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JB Accepts, published online ahead of print on 12 January 2007
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J. Bacteriol. doi:10.1128/JB.01761-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The sugar phosphotransferase system (PTS) of Vibrio fischeri inhibits both magnesium-dependent motility and bioluminescence

Karen L. Visick, Therese M. O'Shea, Adam H. Klein, Kati Geszvain, and Alan J. Wolfe*

Department of Microbiology and Immunology, Loyola University Chicago, 2160 S. First Ave. Bldg. 105, Maywood, IL 60153

* To whom correspondence should be addressed. Email: awolfe{at}lumc.edu.


   Abstract

Magnesium-dependent induction of Vibrio fischeri flagellar biogenesis (Mif) depends upon two diguanylate cyclases, suggesting an inhibitory role for c-di-GMP. Here, we report that cells defective for the sugar phosphotransferase system (PTS) exhibited a magnesium-independent phenotype similar to mutants of the Mif pathway. Unlike Mif mutants, PTS mutants also were hyper-bioluminescent.




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