Identification of the archaeal alg7 gene homolog (N-acetylglucosamine-1-phosphate transferase) of the N-linked glycosylation system by cross-domain complementation in yeast

Institute for Virology, BMFZ, Philipps-University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany; Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, K7L 3N6, Canada

^* To whom correspondence should be addressed. Email: jarrellk{at}.queensu.ca.

	Abstract

The Mv1751 gene product is thought to catalyze the first step in the N-glycosylation pathway in Methanococcus voltae. Here, we show that a conditional lethal mutation in the alg7 gene (N-acetylglucosamine-1-phosphate transferase) in yeast was successfully complemented with Mv1751, highlighting a rare case of cross-domain complementation.