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J. Bacteriol. doi:10.1128/JB.01811-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Polarity in Archaeal Operon Transcription in Thermococcus kodakaraensis

Thomas Santangelo, L'ubomíra uboová, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, and John N. Reeve^*

Department of Microbiology, Ohio State University, Columbus, OH 43210, USA; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto 615-8510, Japan

^* To whom correspondence should be addressed. Email: reeve.2{at}osu.edu.

	Abstract

An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a non-essential -glycosidase in Thermococcus kodakaraensis. Following introduction of nonsense codons into promoter proximal genes, polarity in operon expression has been established in this Archaeon by both microarray hybridization assays and reporter gene expression.

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