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J. Bacteriol. doi:10.1128/JB.01921-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

In Vivo Demonstration of FNR Dimers in Response to Lower O2 Availability

Adrian J. Jervis and Jeffrey Green*

Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK

* To whom correspondence should be addressed. Email: jeff.green{at}sheffield.ac.uk.


  Abstract

Escherichia coli FNR is an O2-sensing transcription factor. In vitro studies indicate that anaerobic iron-sulfur cluster acquisition promotes FNR dimerization. Here two-hybrid assays show that iron-sulfur-cluster-dependent FNR dimers are formed in vivo in response to lower O2 availability, consistent with the current model of FNR activation.


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